The minute-long ad, called "Setting the Record Straight," features Perry alluding to conservative groups like Club for Growth that are hoping to help former Texas Solicitor General Ted Cruz's campaign by airing ads criticizing Dewhurst.
"You know the D.C. insiders are scared when they spend millions of dollars attacking Texas conservatives," Perry says in the ad. "Despite their D.C. double-talk, the facts are simple. David Dewhurst is a conservative fighter."
Perry praises Dewhurst for repeatedly helping to cut state spending and balance the state budget.
Dewhurst isn't the only U.S. Senate candidate in the race using a radio ad to promote a high-profile endorsement. Former Dallas Mayor Tom Leppert released a radio ad last month featuring former Dallas Cowboy Troy Aikman.